Hiraki UHPLC Chromatography column LaChromUltraII C18
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UHPLC The future will begin here.

· 140 MPa System voltage resistance (Hitae High-tech Survey Japanese domestic sales models July 2013) helps you achieve high resolution analysis
· The theoretical number of trays is as high as 50,000 UHPLC Using a high resolution chromatography column ( LaChromUltra II), Help you achieve high resolution analysis
· Total reflective capillary flow cell, helping you achieve high sensitivity and high resolution analysis at the same time
· Take care of both HPLC and UHPLC Analysis system
· MEM Column connector (withstand pressure up to 140 MPa, Optional) and the built-in waste liquid barrel of the system further improve operation
Binary pump adopts new control technology LBT(Liquid Beat Technology ( Liquid compression technology) control technology, from low pressure zone to 140 MPa The high pressure zone is used to carry out stable liquid supply within this wide pressure range. For the gradient analysis of high-pressure zones, good reproducibility analysis results can also be obtained.
In addition, the extremely small system volume structure improves gradient responsiveness and strongly supports ultra-high-speed analysis.
Improve the stability of liquid delivery to achieve UHPLC arrive HPLC Extensive analysis of
use LBT Control technology, corrects the solvent volume elastic coefficient, and the liquid delivery stability is not affected by the liquid delivery pressure and solvent type.
use 5 µm Chromatographic column, 3 µm Chromatographic column, 1.9 µm Stable analysis results can also be obtained when the column is analyzed.

[Conditions] | |
Eluent | 20 mM KH2PO4/CH3CN=95/5 |
Detection | 274 nm |
UHPLC It is considered difficult to conduct high-resolution analysis of related substances and impurities of synthetic compounds such as drugs and chemical synthetic products.
Newly developed UHPLC Using a high resolution chromatography column LaChromUltra II, Combined with sufficient system to withstand pressure 140 MPa, High resolution and high efficiency analysis can be achieved.
High resolution analysis of substances (impurities)
Erythromycin (four-membered cyclic macrolide antibiotic) is used as the separation model sample of the main components and related substances. HPLC Chromatographic column and UHPLC The columns were compared.
HPLC column [Conditions] | |
Column | LaChrom II C18(5 µm) 4.6 mm I.D.×150 mm L. |
Flow rate | 1.0 mL/min |
Eluent | 20 mmol/L Phosphate Buffer/CH3CN/CH3OH=45/40/15 |
Detection | DAD 210 nm |
UHPLC column [Conditions] | |
Column | LaChromUltra II C18(1.9 µm) 3.0 mm I.D.×250 mm L. |
Flow rate | 0.710 mL/min |
Eluent | 20 mmol/L Phosphate Buffer/CH3CN/CH3OH=45/40/15 |
Detection | DAD 210 nm |

Using newly developed UHPLC Using a high resolution chromatography column, better separation results were obtained.
The demand for high sensitivity analysis for harmful substances and genotoxic impurities is increasing. The diode array detector using a total reflective capillary flow cell can meet the needs of higher sensitivity analysis, and also provides an optical path length of 65. mm high sensitivity flow cell.
And it is combined with the double helix mixer and the reduction of sample residues to support high sensitivity analysis.
Total reflective capillary high sensitivity flow cell
Diode array detector ( DAD) The new optical system effectively reduces noise and drift, ensuring high sensitivity analysis results. Use (optional) high sensitivity flow cell (optical path: 65 mm), Higher sensitivity analysis can be achieved.
The sensitivity is approximately the previous model of our company ( LaChromUltra) 10 times more, so it can be applied to samples that require high sensitivity analysis of related substances (impacts), genotoxic impurities, etc. It can be flexibly used for the initial stage of impurity spectrometry control such as raw materials, intermediates, and raw drugs of drugs for drugs and chemical synthetic products.


Quartz glass capillaries are used in the flow channel of the flow cell, and the total reflection of the capillary interface is used to successfully reduce the loss of light transmitted through the flow cell.
Therefore, even extending the optical path length of the flow cell ensures the same baseline performance as before, thereby achieving high sensitivity analysis.
Double helix mixer
Binary pumps are equipped with a new double helix mixer with microfluidics.
Under the premise of low capacity, high mixing performance is achieved, so an extremely stable baseline can be obtained during gradient analysis, achieving higher sensitivity analysis.

The structure of a double helix mixer
The flow path in the mixer is repeatedly branched and combined to achieve high-efficiency solvent mixing. (Mixer Capacity: 55 µL)

[Conditions] | |
Column | LaChromUltra II C18(1.9 µm) 2.0 mm I.D.×50 mm L. |
Eluent | A)0.1% TFA/H2O(v/v) |
Flow rate | 0.500 mL/min |
Detection | UV 214 nm |
Daily maintenance and consumable replacement can be carried out from the front of the instrument, improving operability.
For example, the pump's plunger rod sealing ring, one-way valve spool, the inlet sealing ring of the automatic sampler, the replacement of detector lamps and flow cell, etc., are all used in Hira HPLC Recognized front operation characteristics of the instrument.
LaChromUltra II The organic-inorganic composite silicone material used is physically and chemically better tolerate than the previous silicone particles. To maximize UHPLC High voltage resistance, 1.9 new µm The column length of the particle size is 250 mm The column can not only perform ultra-high speed analysis, but also perform high resolution analysis, thus extending UHPLC Application.
· DAD( Diode array detector) system
· UV-VIS system
Price:Discuss personally
Price:Discuss personally
Price:Discuss personally
Price:Discuss personally
Price:Discuss personally
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