Hiraki UHPLC Chromatography column LaChromUltraII C18
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More than 50 years of manufacturing experience, better performance
Hitachi High Performance Liquid Chromatography Primaide PLUS, The pursuit of higher reliability and durability ensures that you can use it stably for a long time and provide strong support for your daily analysis work.
We recommend you to have more than 50 years HPLC Hitachi products with experience in research and development and manufacturing- Primaide PLUS, Looking forward to becoming your capable assistant.
· Easy operating performance
No matter the size of the laboratory, one person uses multiple units HPLC The situation. Primaide PLUS The design is simple, the components are pre-installed and the maintenance is easy to use even beginners.
· Excellent experimental performance
People are HPLC The requirements for accuracy, reproducibility and stability are increasing day by day. Primaide PLUS Not only meets the limit requirements, but also provides high performance DAD&FL Detectors and other applications have been expanded.
· Reliable durability
Primaide PLUS Adhering to the Rising HPLC Popularly acclaimed durability. Taking into account its excellent performance, each instrument fully complies with Hitachi's strict quality standards.
· Compact design, saving space
The main options such as degassing devices and gradient units are built into the host, and even if the options are added, it does not take up extra space.
· Easy to operate and easy to maintain
All modules (including all types of pipes) can be operated from the front, and the columns can be easily replaced to smooth operation of the flow path system. Taking into account both maintenance, the lamp or circulation pool can be replaced from the front.
· Safe and reliable, liquid leakage-proof
When a fluid leakage occurs in the flow path system, the leakage sensors configured in each module can sense and automatically alarm to improve safety and reliability.
· Integrated control
All modules can be controlled through system management software without the need for an operation panel.
· Fast and reliable communication
Compared with traditional communication, it connects each module to each other, concentrates all communications, realizes the digitization of signals, and improves the reliability and communication rate of communication.
· True and reliable data
Each detector has a built-in mercury lamp, using its characteristic spectral line (254 nm) Automatic verification of ultraviolet wavelengths. Each module has rich quality assurance information to ensure data reliability.

[Standard samples ]
GC: (-)-Gallic catechin (Thermoisomers )、EGC: (-)-epigalactine, C: (-)-Catechins, EGCG: (-)-epigalactine gallate, EC: (-)-epicatechin, GCG: (-)-Gallic catechin gallate (Thermoisomers )、ECG: (-)-epicatechin gallate, CG: (-)-Catechin gallate (Thermoisomers )、Caffeine : caffeine
1110 Pump1210 Automatic sampler1315 Column thermostat1410 UV detector1435 Diode array detector ( DAD)1440 Fluorescence detector1320 Post-column photochemical derivatizerOrganizer
Improve flow rate precision
Hitachi liquid phase pump has always adopted the speed control method to suppress liquid pulses. Primaide PLUS 1110 The pump can monitor pressure fluctuations in real time, set the speed range of the plunger at the optimal time, effectively suppressing liquid pulses.
Improve the reproducibility of gradient analysis retention time
Primaide PLUS 1210 The injection time of the automatic injector is synchronized with the gradient cycle time of the pump, and the retention time reproducibility of gradient analysis is excellent and the analysis accuracy is high.
Gradient reproducibility data | |||
Peak number | Element | Retention time | |
average / min. | R.S.D. / % | ||
1 | Acetylanilide | 2.032 | 0.076 |
2 | Acetosone | 2.793 | 0.080 |
3 | Phenylacetone | 3.581 | 0.096 |
4 | Phenylone | 4.495 | 0.102 |
5 | Benzophenone | 4.809 | 0.098 |
6 | Pentanone | 5.639 | 0.083 |
7 | Benhexanone | 6.975 | 0.082 |
8 | benheptanone | 8.413 | 0.068 |
9 | Octylbenzene | 9.882 | 0.078 |
Measurement conditions | |
Chromatography column | HITACHI LaChrom C18 (5 µm) 4.6 mm I.D.×150 mm L. |
Mobile phase | A : CH3OH B : H2O |
gradient | 0 - 11 min A 65 → 100% |
Column temperature | 40℃ |
flow | 1.0 mL/min |
Test | UV 265 nm |
Injection volume | 10 µL (100 g/mL Each ) |
Direct injection method to save sample volume and improve analysis speed
The direct injection method is adopted. The injection needle is part of the flow path. All samples sucked into the injection needle can be injected into the flow path, reducing sample consumption and giving full play to the advantages in the determination of small and expensive samples.
Compared with the injection method using quantitative rings, the injection steps are simple, the injection cycle can be shortened to half, which improves the measurement speed and helps high-throughput analysis.
High-precision analysis by improving reproducibility
The syringe driving part used to control the sample injection volume adopts a high-precision stepping motor, and the injection time is synchronized with the pump gradient cycle time, improving the reproducibility of the injection volume and the reliability of the analysis results.

Injection volume reproducibility data
Brand new light-proof window design
The new dark window is adopted, with low light transmittance and is compatible with the analysis of light-sensitive samples to ensure good reproducibility of data.

Measurement conditions | |
sample | Methyl parabenzoate |
Mobile phase | CH3OH : H2O = 60 : 40 |
flow | 1.0 mL/min |
Test | UV 265 nm |
Optimize preheating method to achieve sharp peak shape
Through the preheating module with excellent thermal efficiency, the symmetry and sharpness of the chromatographic peaks are improved, and the stability of the analysis results is not affected by ambient temperature changes. The column part adopts air circulation heating to facilitate the replacement of the column.
Standard cooling function
Temperature control range [Ambient temperature-15 ℃(Minimum 4 ℃)】To [ambient temperature +50℃(Up to 65 ℃)】。
A variety of valves are available to meet more needs
Vertical column thermostat has a large space and can be installed with different valves to realize the development of complex samples and the analysis methods.
Low noise and high sensitivity
Primaide PLUS 1410 UV The noise value of the detector is 0.5 ×10-5AU Below, it is particularly suitable for analyzing trace components.
High response and low noise support high-speed analysis
A response time of 0.05 seconds can also achieve low noise, truly detect sharp peak shapes, and conduct high-speed and high-sensitivity analysis.
Stability not susceptible to changes in ambient temperature
We are committed to the constant temperature design of optical systems. Regardless of the customer's laboratory environment, day and night temperature, and temperature changes between seasons, we can obtain stable and excellent reproducibility analysis results.
Automatic wavelength verification of UV light zone through built-in mercury lamp
254 using mercury lamp nm Feature spectrum lines are suitable HPLC Commonly used ultraviolet light zone wavelengths are checked. Cooperate D2The characteristic spectrum of the lamp can be verified to 3 wavelengths, so high reliability analysis can be performed over a wide wavelength range.
Supports high sensitivity analysis
Low noise is further achieved through the adoption of new optical systems (≤0.5 X 10-5AU(250 nm/600 nm))。“DAD The detector has poor sensitivity" - there is no need to be confused by this traditional concept.
Structures that are not susceptible to fluctuations in measured ambient temperatures
The large lamp room is adopted to achieve the stabilization of the light source. The light splitter is separated from the lamp chamber and is insulated, and only cools the lamp chamber, improving the adaptability to ambient temperature changes. The built-in temperature sensor uses a fan that controls the air volume synchronously with the temperature change, which further reduces the temperature fluctuations in the instrument, thereby ensuring low noise, high stability and ensuring the reliability of the analysis results.
High spectral resolution
Configure 1,024-bit diodes for high spectral resolution (0.78 nm/1element). Using a grating as a spectral splitter, the same spectral resolution can be obtained from the ultraviolet to the visible light area.
High-speed analysis through digital noise correction processing
Short data acquisition intervals (0.05 seconds) can also achieve low noise, and sharp peak shapes can also be obtained through low noise in high-speed analysis.
Equipped with automatic wavelength verification function
Automatic wavelength verification of the UV light area is performed through the built-in mercury lamp.

Three-dimensional chromatography
· High sensitivity
The optical system adopts a three-dimensional optical axis configuration optical design, Hitachi's unique condenser, a small volume flow cell and an optimized transmitted light monitoring technology to reduce the loss of lamp energy.
· Fluorescence detector for variable slits
Emitting light monochromator slit can be at 15 nm and 30 nm Choose between, use 30 nm Slits can be used for higher sensitivity analysis.
· Automatic wavelength correction
254 using mercury lamps nm Feature spectrum line pair HPLC Commonly used ultraviolet light zone wavelengths are checked, and the wavelength accuracy is high.
· Excellent photochemical derivatization properties
The heat dissipation system is designed internally to ensure the long-term and stable operation of the analysis system.
The specially designed internal optical path and reaction tube further improve the derivative efficiency and detection sensitivity.
· have LAMP OFF Function
It has a light source monitoring indicator, which can check the light source status at any time.
have LAMP OFF Function, which can automatically turn off the lights after the sequence operation is completed, saving the life of the light source.
The tissue can be placed in multiple solvent bottles
The following solvent bottles can be placed in the tissue device at the same time.
Specific examples: | |
1 | 1000mL×4 |
2 | 500mL×9 |
Organizer is available for power
It can be 1 pump, 1 automatic sampler, 1 column thermostat, 1 detector (any one of 1 ultraviolet detector, 1 diode array detector) and 1 block IFC The interface board is powered.
Price:Discuss personally
Price:Discuss personally
Price:Discuss personally
Price:Discuss personally
Price:Discuss personally
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