Product Description

1、CRY-3B The cell fusion device is suitable for cell hybridization, cell fusion, and can directly observe cells into strings under an inverted microscope. The fusion phenomenon can be used in microbiology, veterinary medicine, bioengineering and other research.

Compared with traditional chemical fusion methods, it has the characteristics of non-toxicity to cells, high fusion efficiency, and convenience and rapidity. Various flat electrodes, needle electrodes and nickel wire electrodes are available.

2、The system adopts high-performance 32-bit ARM Main control chip, fully digital design, LCD The touch screen has a beautiful appearance and is convenient and reliable operation. It is an ideal tool for various colleges and universities and scientific research institutions to carry out cell fusion teaching and scientific research.

Electrical principle of cell fusion instrument: This machine mainly generates alternating current (serial) pulse voltage and direct current (fusion) single pulse voltage.

3、High-frequency AC pulses generated by AC (serial) pulse signal generation circuit (30KHz-3000kHz48vp-p） It can polarize the protoplasts and quickly arrange them in beads.

DC (fusion) single pulse generation circuit can issue DC high-voltage pulses with adjustable pulse width according to the set number of times (5US-5000US、0-700V)Make the electric field intensity between the fusion electrodes reach up to 6000 instantaneously V/CM。

4、Cell electrical fusion mainly uses an alternating current field of frequency and voltage of a certain value to make the protoplasts between two parallel electrodes arranged in beads at certain intervals.

Then, a certain value of high-voltage DC pulse electric field is used to cause reversible breakdown on the cell membrane, thereby inducing the fusion of cells in contact with each other.

5、Because the cells can be polarized to form dipoles under the action of the alternating current pulse of the external electric field, adjacent cells will get closer to each other due to the attraction of static electricity.

At the same time, due to the uneven electric field generated between the electrodes of the fusion chamber, the cells move to electrode parts with high electric field strength in sequence, and are arranged in beads. This arrangement is reversible.

After the electric field disappears, the cells will return to a random distribution state by themselves. In the uneven electric field, the pressure on the cell membrane is unevenly distributed, resulting in local reversible breakdown in the protoplastic cell membrane and forming micropores.

Automatically close after the electric field disappears, but if the electric field intensity exceeds the critical value, irreversible breakdown will occur.

Product Features

1、Multifunction: It has both cell electrofusion and electroporation functions

2、Flexibility: With wide voltage and pulse time range

3、Quick and efficient: It simply and quickly completes the entire process of cell arrangement, fusion and post-fusion processing, in just a few seconds

4、Brand new LCD touch operation 

5、Simple and convenient parameter setting

6、Application areas

1）Cell fusion (the fusion process can be observed under an inverted microscope)

2）Nuclear transfer

3）Embryo operation

4）Hybridoma generation

5）Plant protoplast fusion

6）Gene import in chicken embryos

7）Live genes /Drug import

Technical parameters