Hitachi Fluorescence Spectrophotometer F-7000
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Hitachi Fluorescence Spectrophotometer F-7100 It's in the previous generation model F-7000 The basic performance has been further improved. Sensitivity is one of the important factors in choosing a fluorescence spectrophotometer. F-7100 Compared with previous generation models, the sensitivity is 1.5 times higher, and the Raman signal-to-noise ratio of water is as high as above 20,000. at the same time, F-7100 It uses long-life xenon lamps, which can last up to 5 times longer than the consumable xenon lamps used in previous generation models. F-7100 Configure the fluorescent fingerprint measurement function and the daily difference change correction function to make the operation easier.

Example of determination of trace fluorescein regions
The left image indicates Blank( Pure water) is a reference, ultra-high sensitivity detection example. use F-7100 Detectable as low as 1 ×10-13mol/L( subpimole) fluorescein of the order of magnitude. From the linear curve diagram, we can see that even in the ultra-triple area, good linear relationships can be obtained.

Comparison of lamp service life
F-7100 The new xenon lamp is adopted and the lamp power is improved, which improves the brightness of the light source and effectively extends the service life of the xenon lamp. Extending the light bulb replacement cycle can reduce the cost of consumables and reduce the operating burden of light bulb replacement (as shown in the picture on the left above). As can be seen from the picture above, F-7100 Excellent performance, even if xenon lamp is used close to 2500 h, It can also maintain high sensitivity.

Three-dimensional time-scanned fluorescence spectrogram of coumarin in environmental applications
Traditional fluorescence spectrometers are difficult to achieve chemical reaction monitoring, but F-7100 Ultra-high speed scanning speeds make this measurement easy. In this example, the fluorescence spectroscopy measurement tracks the isomerization process of coumarin in kerosene over a 2-second interval and is displayed in a three-dimensional time scan spectrum.
Previously, rapid chemical reactions that occurred within 1 minute could only be monitored by a fixed wavelength method. because F-7100 The fast scanning capability can complete measurements of the entire wavelength range within 1 second, so it can track such a fast reaction process.

Fluorescence intensity standardized screen
The slight difference in intensity caused by fluorescence due to different test dates can be corrected.
The fluorescence intensity will be affected by changes in the brightness of xenon lamps, room temperature changes, optical systems, etc. Use the fluorescence standardization function to convert the fluorescence intensity of the sample with the fluorescence intensity of the standard sample. This function makes the fluorescence peak intensity values tested by different instruments at different times comparable.
project | content |
Sensitivity | S/N 1,200 above( RMS)*1 |
Metering method | Calculation method of monochrome light monitoring ratio |
light source | 150W Xe Automatic deozooming of lamps |
Wavelength scanning speed | 30、60、240、1,200、2,400、12,000、30,000、60,000 nm/min |
Wavelength movement speed | 60,000 nm/min |
Wavelength range | 200-750 nm, Zero-level optical |
Spectral bandwidth | Excitation side: 1, 2.5, 5, 10, 20 nm |
Resolution | 1.0 nm (546.1 nm Where ) |
Wavelength accuracy | ±1 nm Within |
Three-dimensional detection time | 3min Within *3 |
*1Excitation wavelength 350 nm, Slit 5 nm, Response time 2 s
*2Excitation wavelength 350 nm, Slit 10 nm, Response time 4 s
*3Excitation wavelength 350-750 nm, Sample interval 10 nm
Emission wavelength 350-750 nm, Sample interval 10 nm
Sample pool holder | Solid sample holder | Suitable for the detection of solid samples, powder samples, and high concentration solutions. |
Absorption spectrometry bracket | Absorption spectroscopy can be performed. | |
High sensitivity sample pool holder | By using a spherical mirror, the sensitivity is increased by about 2 times. | |
Micro sample pool holder | Micro sample detection can be performed. | |
Filter・Gloss reduction board | Automatic filter accessories | A spectrum without high order light can be obtained. |
Filter assembly | High order light caused by scattered light can be filtered. | |
Light reduction plate assembly | It can reduce the luminous intensity of the bright light-emitting sample. | |
Fluorescence polarization | Manual polarization accessories | Suitable for UV /Polarity detection of visible light areas and visible light areas. |
Automatic polarization accessories | It can automatically measure fluorescence polarization and fluorescence anisotropy. | |
Multiple sample testing | Sample suction device accessories | It can realize online sample suction, detection and data output. |
8Pool conversion sample holder | Can replace up to 8 10 mm Square container, high quantitative analysis speed of samples. | |
4Pool conversion sample holder | Up to 4 10 can be replaced mm Square container, high quantitative analysis speed of samples. | |
Automatic 4-cell conversion sample constant temperature pool | Up to 4 10 can be automatically switched mm Square container, high quantitative analysis speed of samples. | |
Microplate accessories | It can automatically detect up to 96 samples, and the injection volume is only 300 µL。 | |
Temperature regulation | Water bath constant temperature bracket | Suitable for biochemistry and other sample analysis. |
Water bath constant temperature stirring bracket | The samples can be stirred to achieve high-temperature and high-precision detection. | |
Low temperature test accessories | Low temperatures can be achieved (-196 °C) Detect fluorescence and phosphorescence. | |
Electronic constant temperature sample pool holder constant temperature control | It can detect samples in a constant temperature, especially suitable for biological sample detection. | |
Electronic constant temperature sample pool holder program control | The sample temperature can be set through the program and the constant temperature detection can be maintained. | |
Intracellular Ca Ion detection | Intracellular Ga Ion detection accessories | Used in cells Ga Ion detection. |
Micro-sampling device | Can be used to monitor reaction changes after reagent addition. | |
Intracellular Ga Ion detection procedure | Can detect intracellular Ga Ion concentration. | |
Sample pool | Micro sample pool | High sensitivity analysis of micro samples can be performed. |
Low scattering micro sample pool | It uses black quartz and has less scattered light. | |
Quartz fluorescent sample pool | In UV /Quartz with light transmittance is used in the visible light area. | |
Fluorescent-free sample pool | It can reduce background fluorescence and is very suitable for low-concentration sample detection. | |
other | Photomultiplier tube | The detection wavelength range can be extended to 200 ~900nm。 |
Substandard light source | Long wavelength spectral correction can be achieved. | |
LC Micro circulation tank | The sample volume is 55 µL and 180 µL Optional. | |
Quantum yield detection unit | The built-in integration sphere can directly detect the quantum yield of solid samples, transmission measurement of fluorescent samples, and fluorescence measurement of transmission direction. |
Long-life xenon lamps improve customer maintenance experience, optimal sensitivity, and achieve low noise measurement;
Quickly measure samples of different forms such as liquids and solids without complex pretreatment;
Qualitative quantitative analysis such as sample type origin judgment, mixing ratio calculation, and detection of harmful substances can be carried out.;
At the same time‘ EEM Assist’ Program package, supports standardized analysis, fluorescent fingerprint batch output and other functions;
It will save you more time and experience the simplicity of analysis.
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